Methods using different techniques of collagenase digestion and discontinued density gradient centrifugation , the adult swine and rat islets were prepared 方法采用不同的胶原酶消化法及不连续密度梯度纯化法。
So a lagre populations of relatively purified spermatids can be isolated from mouse testis by enzymatic digestion combined with discontinuous percoll gradient centrifugation method 因此采用组合酶消化结合非连续percoll梯度离。 g法,可有效地从小鼠睾丸中分离到较多较纯的存活精子细胞。
Methods : gradient centrifugation and differential adherence were employed to isolate type pneumocytes from the lungs in the rats , proliferative activities were compared in the cultued cells from the young , adult and aged rats 方法:应用梯度离心和贴壁培养分离大鼠肺泡型细胞,比较幼年、中年和老年大鼠培养肺泡型细胞的增殖能力。